Sperm DNA fragmentation testing: proceed with care
نویسندگان
چکیده
tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The authors should be commended for their thorough review of DNA fragmentation and the clinical indications for testing. The basic science review of DNA fragmentation, oxidative stress, and its association with male infertility is detailed yet concise. The authors also present clinical scenarios to better illustrate the current role of evaluating sperm DNA fragmentation (SDF) in the infertility specialist’s armamentarium, and describe very convincing theoretical arguments for the potential importance of SDF. However, the clinical role of SDF in the evaluation of men with male factor infertility remains unclear, and several recommendations made in this article do not yet have sufficient clinical data to support its routine use.
منابع مشابه
P-81: Evaluation of DNA Fragmentation in Patients with Total Globozoospermia
Background: Infertility is estimated to affect up to 15% of couples of reproductive age, and male factors contribute to 50% of these cases. Globozoospermia syndrome is a severe form of teratozoospermia, with an incidence of less than 0.1%. It is characterized by round sperm head. The most prominent feature of globozoospermia is the malformation of the acrosome, which is totally absent in the to...
متن کاملP-19: Effect of Three Sperm Preparation Methods on Sperm DNA Integrity and Oocyte Fertilization Rate
Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...
متن کاملThe relationship between sperm viability and DNA fragmentation rates
BACKGROUND In humans, sperm DNA fragmentation rates have been correlated with sperm viability rates. Reduced sperm viability is associated with high sperm DNA fragmentation, while conversely high sperm viability is associated with low rates of sperm DNA fragmentation. Both elevated DNA fragmentation rates and poor viability are correlated with impaired male fertility, with a DNA fragmentation r...
متن کاملStudying of the Expression of BAX and BCL-2 Genes in Men with Sperm DNA Fragmentation Based on DFI and Comet Assay
Introduction: The amount of expression of BAX and BCL-2 genes in infertile men’s sperm as well as its association with sperm parameters and DNA fragmentation index is an issue which has not been studied yet. In this research, it is assumed that up-regulation of BAX and downregulation of BCL-2 are directly associated with sperm DNA fragmentation. Methods: After obtaining semen samples from the ...
متن کاملThe effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men
Background: One of the main causes of male infertility is the negative effects of oxidative stress. Follicle-stimulating hormone (FSH) plays an essential role in spermatogenesis, as well as in the maintenance of sperm DNA integrity. This study aimed to determine whether the recombinant human follicle-stimulating hormone (rhFSH) treatment of sperm parameters could positively affect sperm DNA and...
متن کامل